PE Rabbit anti-Mouse CD79b Monoclonal Antibody for FC - P15530

Core Product Specifications and Parameters

Biological Background: CD79b Function and Localization

• CD79b, also known as B-cell-specific glycoprotein B29 or Ig-beta, is a transmembrane protein that forms the beta chain of the B-cell antigen receptor complex (BCR).
• It functions in cooperation with CD79A to initiate signal transduction upon BCR activation, leading to internalization of the complex, trafficking to late endosomes, and antigen presentation.
• CD79b enhances phosphorylation of CD79A, likely by recruiting kinases or protecting CD79A from dephosphorylation, amplifying BCR signaling.
• The protein is primarily expressed on the surface of B-cells and is essential for adaptive immunity.
• Subcellular localization is the cell membrane, where it participates in receptor-mediated signaling.
• Post-translational modifications include phosphorylation, glycosylation, and disulfide bond formation, which may regulate its function and stability.
• CD79b contains an immunoglobulin domain and functions as a receptor, highlighting its role in antigen recognition and immune response.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 26–158 of mouse CD79b, corresponding to the extracellular domain; thus, the antibody is expected to detect surface-expressed CD79b on live B-cells in flow cytometry.
• As a PE-conjugated antibody, ensure proper compensation and use appropriate isotype controls to minimize background in multicolor panel experiments.
• Validate the antibody’s performance under your specific experimental conditions, including optimal titration and staining protocols, as performance may vary with cell type and sample preparation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with seamless access to a comprehensive portfolio of IVD raw materials. For B-cell immunophenotyping and BCR signaling studies, we supply validated monoclonal antibodies, matched antibody pairs, and ancillary reagents to streamline your workflow. Trust CamelBio as your reliable partner for high-quality raw materials, from concept to clinical application.