PE Rabbit anti-Mouse CD70 mAb for FC - O55237

Core Product Specifications and Parameters

Biological Background: CD70 Antigen Function and Localization

• CD70 antigen, also known as CD27 ligand (CD27-L) and tumor necrosis factor ligand superfamily member 7 (TNFSF7), is a type II transmembrane glycoprotein belonging to the TNF ligand family.
• It serves as the ligand for the CD27 receptor, which is specifically expressed on the surface of T cells. The CD70-CD27 signaling pathway plays a critical role in mediating antigen-specific T cell activation and expansion, thereby providing immune surveillance of B cells.
• CD70 is primarily localized at the plasma membrane, consistent with its function as a membrane-bound ligand.
• Tissue expression is normally very low; it has been detected in splenocytes and thymocytes.
• The protein contains a disulfide bond, a transmembrane helix, and a signal-anchor, and it is glycosylated.

Experimental Guidance and Technical Tips

• For flow cytometry (FC), perform antibody titration to determine the optimal concentration for your specific cell type and experimental conditions.
• Include appropriate isotype controls and fluorescence-minus-one (FMO) controls to ensure specificity of CD70 staining.
• Since the immunogen corresponds to the extracellular domain (amino acids 47-195), this PE-conjugated antibody is suitable for surface staining of live cells.
• Consider validating the antibody in your target cell populations, as CD70 expression is typically very low and may require enrichment or stimulation for detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. For CD70-related research and assay development, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement. Whether you need a PE-conjugated anti-CD70 mAb for flow cytometry or complementary reagents for functional studies, CamelBio streamlines your supply chain with reliable quality and technical support.