PE Rabbit anti-Mouse CD68 Monoclonal Antibody for Flow Cytometry (Intracellular) - P31996

Core Product Specifications and Parameters

Biological Background: Macrosialin (CD68) Function and Localization

• Macrosialin (CD68), also known as Lamp4, gp110, or Scard1, is a heavily glycosylated transmembrane protein belonging to the lysosomal-associated membrane protein family.
• It plays a critical role in phagocytic activities of tissue macrophages, participating in intracellular lysosomal metabolism and mediating extracellular cell-cell and cell-pathogen interactions.
• CD68 binds to tissue- and organ-specific lectins or selectins, facilitating the homing of macrophage subsets to particular anatomical sites.
• Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane enables macrophages to crawl over selectin-bearing substrates or other cells, aiding in immune surveillance.
• The protein localizes predominantly to endosome and lysosome membranes, but can also be expressed on the cell surface.
• It is expressed primarily in tissue macrophages and, to a lesser extent, in dendritic cells.
• Post-translational modifications include glycosylation and disulfide bond formation; the protein undergoes alternative splicing, generating multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 21–291 of mouse CD68, corresponding to the luminal domain; fixation and permeabilization are required for intracellular staining.
• Fluorescence-conjugated anti-CD68 antibodies are commonly used for flow cytometry to phenotype macrophages; consider using Fc receptor blocking and isotype controls to minimize non-specific binding.
• The PE conjugate provides bright signal and is suitable for multicolor panels; titrate the antibody in the relevant sample system to optimize concentration.
• Validate detection sensitivity in the biological context of interest, as CD68 expression levels can vary with macrophage activation state.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to streamline IVD raw material procurement, from concept to clinic. This PE-conjugated anti-CD68 antibody supports macrophage and dendritic cell research, and we can further supply validated antibody pairs, recombinant proteins, and ancillary reagents. For rare-target or custom antibody development needs, CamelBio's sourcing network ensures timely access to high-quality materials, accelerating your immunological assay development.