PE Rabbit anti-Mouse CD62L Monoclonal Antibody for Flow Cytometry - P18337

Core Product Specifications and Parameters

Biological Background: CD62L (L-selectin) Function and Localization

• CD62L, also known as L-selectin, is a calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells. It plays a key role in lymphocyte homing to peripheral lymph nodes and leukocyte tethering/rolling on endothelium. Related references: PMID:1693096
• The protein is expressed on the surface of most leukocytes and is predominantly found in lymphoid tissues.
• L-selectin is a transmembrane protein with an extracellular region containing a C-type lectin domain, an EGF-like domain, and multiple sushi (SCR) repeats. The cytoplasmic tail is involved in signaling.
• It is rapidly shed from the cell surface upon leukocyte activation, a process mediated by proteolytic cleavage.
• CD62L belongs to the selectin family of adhesion molecules, which also includes E-selectin and P-selectin.
• Post-translational modifications include glycosylation and disulfide bond formation essential for ligand binding.

Experimental Guidance and Technical Tips

• This antibody is raised against the extracellular domain (aa 39-332) and is PE-conjugated, making it suitable for direct flow cytometric analysis of surface CD62L expression on live mouse cells.
• For optimal staining, consider titrating the antibody to determine the best concentration for your specific cell type and flow cytometry conditions.
• Use an appropriate isotype control and Fc receptor blocking to minimize non-specific binding.
• Note that PE is a bright fluorophore; avoid prolonged light exposure to prevent photobleaching.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw-material procurement, offering a comprehensive range of validated antibodies, antigen pairs, and ancillary reagents to support diagnostic development from concept to clinic. This high-quality PE anti-CD62L monoclonal antibody is an ideal tool for immunological research and clinical marker analysis. For bulk orders, custom conjugations, or assistance with rare target sourcing, our expert team is ready to support your project needs.