PE Rabbit anti-Mouse CD62E Monoclonal Antibody for FC - Q00690

Core Product Specifications and Parameters

Biological Background: E-selectin Function and Localization

• E-selectin, also known as CD62E, ELAM-1, or LECAM2, is encoded by the Sele gene and functions as a cell-surface glycoprotein involved in immunoadhesion.
• It mediates the tethering and rolling of blood neutrophils on cytokine-activated endothelium through interaction with SELPLG/PSGL1, facilitating leukocyte recruitment to inflammatory sites. Related references: PMID:1375914
• Belongs to the selectin family of adhesion molecules, characterized by an N-terminal C-type lectin domain, an EGF-like domain, multiple sushi (SCR) repeats, a transmembrane helix, and a short cytoplasmic tail.
• Localizes primarily to the cell membrane of endothelial cells upon stimulation by pro-inflammatory cytokines.
• Structurally, E-selectin is a calcium-dependent (C-type) lectin, with disulfide bonds stabilizing its extracellular domains and N-linked glycosylation contributing to its mature molecular weight.
• Additional roles in capillary morphogenesis have been suggested, though the precise mechanisms remain less defined.

Experimental Guidance and Technical Tips

• This PE-conjugated monoclonal antibody is specifically indicated for flow cytometry (FC), allowing direct detection of mouse CD62E on the cell surface without a secondary antibody.
• The immunogen encompasses a large extracellular region (aa 22–557), suggesting the antibody may recognize the native, properly folded protein; consider epitope accessibility when designing experiments.
• For optimal FC staining, titrate the antibody to determine the appropriate working concentration and include appropriate isotype controls. Validate performance in your specific experimental system (e.g., activated primary endothelial cells or transfected cell lines).
• Although not validated for other applications, the antibody may be suitable for imaging or functional assays; empirical testing with proper controls is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers, research laboratories, and diagnostics developers with efficient access to premium raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This PE Rabbit anti-Mouse CD62E monoclonal antibody supports studies in endothelial cell biology and inflammation, and our team can assist with sourcing complementary reagents or custom conjugation services to streamline your experimental workflows.