PE Rabbit anti-Mouse CD6 mAb - Q61003

Core Product Specifications and Parameters

Biological Background: CD6 Function and Localization

• CD6 is a T-cell differentiation antigen and a type I transmembrane glycoprotein belonging to the scavenger receptor cysteine-rich (SRCR) superfamily.
• It mediates cell-cell contacts by binding to ALCAM/CD166, functions as a costimulatory molecule that promotes T-cell activation and proliferation, and contributes to the formation and maturation of the immunological synapse.
• Contributes to signaling cascades triggered by activation of the TCR/CD3 complex. Related references: PMID:24584089
• Functions as a calcium-dependent pattern recognition receptor that binds both Gram-positive and Gram-negative bacteria via lipoteichoic acid and lipopolysaccharide (LPS). LPS binding activates downstream MAP kinases, leading to pro-inflammatory cytokine secretion.
• Localized to the cell membrane.
• Predominantly expressed in thymus, lymph node, and spleen.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation.
• Alternative splicing generates multiple isoforms with predicted molecular weights of 64, 68, and 72 kDa.

Experimental Guidance and Technical Tips

• The immunogen (aa 20–155) targets the extracellular domain, making it well-suited for flow cytometry (FC). Titrate the antibody to determine the optimal dilution and include appropriate isotype controls.
• CD6 is highly expressed in T-cell–rich tissues such as thymus, lymph node, and spleen; these serve as reliable positive controls.
• PE conjugation enables direct detection without a secondary antibody; follow standard FC protocols.
• Validate specificity using CD6 knockout or knockdown models if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner providing diagnostic manufacturers, laboratories, and research institutes with one-stop access to validated antibodies, bulk ancillary reagents, and consulting services across the development pipeline. For CD6-related T-cell and inflammation research, we offer comprehensive sourcing of rare-target raw materials, from monoclonal/polyclonal antibodies to supporting reagents. Trust CamelBio to streamline your supply chain and accelerate your project from concept to clinic.