PE Rabbit anti-Mouse CD50/ICAM-3 (ICAM-5/Telencephalin) Monoclonal Antibody for FC - Q60625

Core Product Specifications and Parameters

Biological Background: CD50/ICAM-3 (ICAM-5/Telencephalin) Function and Localization

• The target protein, intercellular adhesion molecule 5 (ICAM-5), also known as telencephalin, is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily.
• ICAM-5 functions as a cell adhesion molecule, serving as a ligand for the integrin LFA-1 (ITGAL:ITGB2), thereby mediating neuron–leukocyte adhesion. Related references:
• ICAM-5 promotes dendritogenesis and neuronal arborization in hippocampal neurons through homophilic cell adhesion, essential for proper synaptic connectivity. Related references: PMID:22187327
• It is exclusively expressed in telencephalic regions of the brain, highlighting its specialized role in forebrain neural circuits.
• Subcellularly, ICAM-5 localizes to the plasma membrane and is enriched in dendritic spine membranes and uropodium, positioning it at sites of synaptic contact and immune surveillance.
• Post-translational modifications include phosphorylation and disulfide bond formation, which may regulate its adhesive properties and membrane dynamics.

Experimental Guidance and Technical Tips

• The PE conjugate and monoclonal format make this antibody suitable for direct flow cytometry detection of surface ICAM-5 on mouse cells without the need for secondary reagents.
• Given the exclusive telencephalic expression, consider using brain tissue-derived single-cell suspensions or cultured primary neurons for validation; test both fresh and fixed samples for optimal staining.
• The immunogen corresponds to the N-terminal extracellular region (aa 32–227), ensuring recognition of surface-exposed epitopes; verify performance under native and denaturing conditions if expanded applications are desired.
• Include appropriate isotype controls and Fc receptor blocking steps to minimize non-specific binding in flow cytometry assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing platform for IVD raw materials, serving diagnostic manufacturers and research labs worldwide. With a focus on neuroscience and cell adhesion targets like ICAM-5, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. From rare target sourcing to technical consultation, CamelBio accelerates your journey from concept to clinic.