PE Rabbit anti-Mouse CD5 Monoclonal Antibody for FC - P13379

Core Product Specifications and Parameters

Biological Background: CD5 Function and Localization

• CD5 is a lymphoid-specific receptor expressed on all T-cells and a subset of B-cells (B1a), playing a pivotal role in T-cell and B-cell homeostasis and immune tolerance.
  Related references: PMID:35720357

• It mediates contact-dependent T-cell help for B-cell activation by engaging ligands such as CD5L or CD72, and maintains regulatory T and B-cell balance.
  Related references: PMID:24950378, PMID:9064341, PMID:9723705

• During thymocyte development, CD5 functions as a negative regulator of TCR signaling, associating with LCK, CD3ζ, PI3K, and CBL to modulate signal strength.
  Related references: PMID:11157848

• Co-engagement of CD3 with CD5 enhances CBL recruitment, promoting VAV1 phosphorylation and degradation, which dampens downstream TCR signaling.

• In B-cells, CD5 modulates IL-10 production through a Ca²⁺-dependent, TRPC1-mediated pathway leading to ERK1/2 activation.

• CD5 is a type I transmembrane protein with a predicted molecular weight of ~54 kDa, localized at the cell membrane. Post-translational modifications include disulfide bonds, glycosylation, and phosphorylation, consistent with its receptor and signaling functions.

Experimental Guidance and Technical Tips

• The immunogen comprises the extracellular domain of mouse CD5 (amino acids 25–371), providing excellent recognition of the native receptor on live cells for flow cytometry applications.
• As a directly conjugated PE antibody, it is ready-to-use in multicolor flow cytometry panels, eliminating the need for secondary staining.
• Titrate the antibody to determine the optimal staining index for your specific cell type and experimental conditions, and validate in the appropriate murine tissue or cell samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated provider of IVD raw materials, technical services, and consulting—supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. This PE anti-mouse CD5 monoclonal antibody exemplifies our commitment to supplying high-quality reagents for immunology and cell therapy research. Our comprehensive sourcing capabilities include validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement to accelerate your diagnostic development.