PE Rabbit anti-Mouse CD47 Monoclonal Antibody for FC - Q61735

Core Product Specifications and Parameters

Biological Background: Leukocyte Surface Antigen CD47 Function and Localization

• Adhesive protein that mediates cell-to-cell interactions and acts as a receptor for thrombospondin THBS1, modulating integrin signaling through heterotrimeric G proteins (By similarity).
• Involved in diverse processes including signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation (PubMed:20610415, PubMed:23591719, PubMed:27742621). Related references: PMID:20610415, PMID:23591719, PMID:27742621
• Plays a role in pulmonary endothelin EDN1 signaling and nitric oxide (NO) signaling, supporting blood pressure regulation (PubMed:27742621, PubMed:20610415). Related references: PMID:27742621, PMID:20610415
• Positively modulates FAS-dependent apoptosis in T-cells (PubMed:15917238) and inhibits stem cell self-renewal via regulation of key transcription factors (PubMed:23591719). Related references: PMID:15917238, PMID:23591719
• Suppresses angiogenesis and may be involved in metabolic dysregulation during aging (PubMed:32679764); negatively modulates wound healing in response to THBS1 (PubMed:18156939). Related references: PMID:32679764, PMID:18156939
• Receptor for SIRPA and SIRPG, influencing dendritic cell maturation, T-cell proliferation, and immunoregulation (By similarity).
• May prevent premature elimination of red blood cells (PubMed:10856220) and plays a role in memory formation and synaptic plasticity in the hippocampus (By similarity). Related references: PMID:10856220
• Localized at the cell membrane and features immunoglobulin domain, disulfide bonds, glycosylation, and transmembrane region.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal extracellular domain (aa 19-135), suggesting reactivity with native surface CD47 in live-cell flow cytometry.
• PE conjugation enables direct detection; consider optimizing antibody concentration to achieve optimal signal-to-noise ratio and include appropriate isotype controls.
• As CD47 is expressed on various hematopoietic and non-hematopoietic cells, validate staining in the target cell population of interest.
• For multicolor panels, ensure minimal spectral overlap with other fluorophores used.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. For CD47-driven research in immune regulation and cell adhesion, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and support rare-target raw material sourcing—bridging your development from concept to clinic.