PE Rabbit anti-Mouse CD45.1 mAb - P06800

Core Product Specifications and Parameters

Biological Background: CD45.1 Function and Localization

• CD45.1, also referred to as Leukocyte common antigen (L-CA), Lymphocyte antigen 5 (Ly-5), T200, and B220, is a receptor-type tyrosine-protein phosphatase C encoded by the Ptprc gene in mice.
• It functions as a transmembrane glycoprotein essential for T-cell activation through the antigen receptor, acting as a positive regulator of T-cell coactivation upon binding to DPP4.
• The protein contains two tandem intracellular PTPase domains; the first possesses enzymatic activity, while the second modulates the substrate specificity of the first.
• Upon T-cell activation, CD45.1 recruits and dephosphorylates key signaling molecules including SKAP1, FYN, and LYN, thereby fine-tuning downstream signaling cascades.
• At the immunological synapse, CD45.1 interacts with CLEC10A on antigen-presenting cells to modulate T-cell activation thresholds and help prevent autoreactivity.
• It localizes primarily to the cell membrane, membrane rafts, and the immunological synapse, consistent with its central role in immune cell communication.
• Post-translational modifications include extensive glycosylation and phosphorylation, contributing to functional regulation and isoform diversity.
• Alternative splicing generates multiple isoforms, reflected in observed molecular weights ranging from approximately 130 to 144 kDa.

Experimental Guidance and Technical Tips

• This PE-conjugated antibody is designed for flow cytometry; consider optimizing the titration and using appropriate isotype controls to ensure specific staining.
• The immunogen corresponds to an extracellular region (amino acids 311–410), suggesting the antibody should detect surface-exposed epitopes on viable cells.
• Validate the antibody in known CD45.1-expressing mouse immune cell populations, such as splenocytes or thymocytes, to confirm expected staining profiles.
• Be aware that CD45.1 exists as multiple isoforms due to alternative splicing; the antibody’s reactivity may vary depending on the isoform expressed in your sample.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your IVD development with a comprehensive portfolio of raw materials, including this PE-conjugated anti-mouse CD45.1 monoclonal antibody for T-cell and immune function research. Our one-stop platform offers access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare-target raw materials. Partner with us to streamline your procurement from concept to clinic.