PE Rabbit anti-Mouse CD44 Monoclonal Antibody for FC - P15379

Core Product Specifications and Parameters

Biological Background: CD44 antigen Function and Localization

• CD44 antigen, also known as Ly-24, Pgp-1, HERMES, extracellular matrix receptor III (ECMR-III), GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Hermes antigen, hyaluronate receptor, lymphocyte antigen 24, phagocytic glycoprotein 1 (PGP-1), and phagocytic glycoprotein I (PGP-I), is a multifunctional cell-surface glycoprotein encoded by the Cd44 gene in mouse.
• CD44 engages extracellular matrix components like hyaluronan (HA), collagen, growth factors, cytokines, and proteases through its ectodomain, and assembles protein complexes via its cytoplasmic domain to facilitate signal transduction, influencing cell migration, adhesion, and activation. Related references: PMID:25065622, PMID:8343954
• Upon LGALS9 ligand binding, CD44 activates downstream signaling components LCK, ERK, and MAPK to promote NK cell activation. Related references: PMID:37006235
• CD44 participates in T-lymphocyte activation, recirculation and homing, hematopoiesis, inflammation, and response to bacterial infection.
• Subcellularly, CD44 localizes to the cell membrane, cell projections (microvilli), and can be secreted.
• In mice, CD44 is expressed in the intestinal epithelium.
• The protein undergoes alternative splicing, glycosylation, phosphorylation, proteoglycan modification, sulfation, and contains disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular region (amino acids 23–176) of mouse CD44, which is well-suited for detecting cell surface CD44 by flow cytometry.
• For flow cytometry, titrate the antibody to determine optimal concentration for your specific cell type, and include appropriate isotype controls to assess background staining.
• Consider confirming reactivity in your model system, as expression levels may vary with cell activation state or tissue source.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a comprehensive one-stop sourcing solution for IVD raw materials. This PE-conjugated anti-mouse CD44 monoclonal antibody supports research in immune cell trafficking, inflammation, and oncology, and is part of our broader portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing capabilities. We are committed to bridging your needs from concept to clinic with reliable supply and technical consultation.