PE Rabbit anti-Mouse CD43 mAb - P15702

Core Product Specifications and Parameters

Biological Background: Leukosialin (CD43) Function and Localization

• Leukosialin (CD43, sialophorin) is a major transmembrane sialoglycoprotein on leukocytes, characterized by a signal peptide, a large extracellular mucin-like domain, a single transmembrane helix, and a cytoplasmic tail. It is heavily O-glycosylated and serves as the predominant cell surface sialoprotein.
• Regulates multiple T-cell functions including activation, proliferation, differentiation, trafficking, and migration. Positively regulates lymph-node trafficking via association with ERM proteins (EZR, RDX, MSN) (PubMed:11728336, PubMed:17638845, PubMed:21289089). Related references: PMID:11728336 PMID:17638845 PMID:21289089
• Negatively regulates Th2 cell differentiation, skewing towards Th1 lineage commitment, and promotes IFN-gamma expression upon TCR activation, thereby influencing cytokine receptor expression and signaling (PubMed:18490738). Related references: PMID:18490738
• Serves as a major E-selectin ligand on Th17 cells, facilitating rolling on activated vasculature and recruitment during inflammation; critical for Th17 but not Th1 cell adhesion to E-selectin (PubMed:26700769). Related references: PMID:26700769
• Acts as a counter-receptor for SIGLEC1 (sialoadhesin) on macrophages and protects cells from apoptotic signals, promoting cell survival (PubMed:11238599). Related references: PMID:11238599
• Localizes primarily to the plasma membrane and cell projections (microvilli, uropodium) but also detected in the nucleus and PML bodies, suggesting roles beyond the cell surface.
• Highly expressed on the surface of thymocytes, T-lymphocytes, neutrophils, plasma cells, and myelomas, consistent with its leukocyte-specific functions.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and staining conditions.
• CD43 is a surface marker; prepare single-cell suspensions and perform surface staining without fixation/permeabilization unless intracellular epitopes are targeted. However, note that some CD43 may be intracellular.
• Use appropriate isotype controls and validate with known positive (e.g., mouse splenocytes or T-cell lines) and negative (e.g., CD43 knockout cells or non-hematopoietic cells) controls.
• Consider that CD43 may be shed or internalized upon activation; optimize staining time and temperature to capture surface expression accurately.
• The immunogen covers the extracellular domain (aa 20-248), ensuring recognition of the native surface protein; however, confirm reactivity in your model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop sourcing partner for IVD raw materials, offering a comprehensive portfolio including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our anti-CD43 antibody supports the development of immunophenotyping and immune function assays. For specialized requirements or scale-up needs, contact our team to explore custom sourcing solutions.