PE Rabbit anti-Mouse CD40 Monoclonal Antibody for Flow Cytometry - P27512

Core Product Specifications and Parameters

Biological Background: CD40 Function and Localization

• CD40 is a member of the tumor necrosis factor receptor superfamily and functions as the receptor for CD40 ligand (TNFSF5/CD40LG).
• Ligand engagement triggers intracellular signaling cascades involving TRAF6 and MAP3K8, leading to ERK activation (PubMed:12881420). Related references: PMID:12881420
• This TRAF6/MAP3K8-mediated ERK signaling is essential for B cell activation and immunoglobulin secretion.
• CD40 is predominantly localized at the cell membrane, but alternative splicing generates a secreted isoform.
• The protein is a glycoprotein with a signal peptide, disulfide bonds, and a transmembrane domain.
• CD40 plays a critical role in adaptive immunity, particularly in T cell-dependent B cell responses.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (amino acids 20-189), making this antibody suitable for detecting cell surface CD40 by flow cytometry.
• For flow cytometry, consider titrating the antibody to optimize staining concentration for your specific cell type and experimental setup.
• Use an appropriate PE-conjugated isotype control and viability dye to ensure specificity and exclude dead cells.
• Validate staining on mouse B cells or macrophages, where CD40 expression is well-characterized.
• When multiplexing with other fluorophores, ensure proper compensation is applied to avoid spectral overlap.
• Store the antibody protected from light at 2-8°C and avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing platform for IVD raw materials, supporting researchers and diagnostic developers from concept to clinic. We supply validated antibodies, antigens, and ancillary reagents for immune research, including monoclonal antibodies like this anti-CD40 clone. Whether you need routine supplies or customized rare-target sourcing, our expert team ensures seamless procurement and technical support.