PE Rabbit anti-Mouse CD352/SLAMF6 Monoclonal Antibody for Flow Cytometry - Q9ET39

Core Product Specifications and Parameters

Biological Background: CD352/SLAMF6 Function and Localization

• SLAM family member 6 acts as a self-ligand receptor modulating activation and differentiation of immune cells via homo/heterotypic interactions, with activity controlled by adaptors SH2D1A/SAP and SH2D1B/EAT-2. Related references: PMID:19648922
• Triggers cytolytic activity in NK cells with high natural cytotoxicity receptor density; positive signaling involves VAV1 phosphorylation and depends on SH2D1B.
• Regulates thymic NKT cell lineage development together with SLAMF1. Related references: PMID:18031695
• Promotes Th17 differentiation and IL-17 secretion via SH2D1A-dependent costimulation, recruiting RORC to the IL-17 promoter.
• May negatively regulate humoral immune response with SLAMF1 and CD84; negatively regulates germinal center formation by inhibiting T-cell:B-cell adhesion via increased PTPN6/SHP-1 association in absence of SAP. Related references: PMID:25926831, PMID:22683125
• Involved in T-cell:B-cell adhesion and maintaining B-cell tolerance in germinal centers, preventing autoimmunity. Related references: PMID:20153220, PMID:25801429
• Isoform 3 may suppress pathogenic T-cell proliferation. Related references: PMID:21422172
• Subcellular localization: Cell membrane.
• Expressed on hematopoietic cells; isoform 3 in thymocytes and B lymphocytes of C57Bl/6 strain.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 31-235), suggesting recognition of the native surface receptor; no permeabilization required for flow cytometry staining of live cells.
• PE conjugation enables direct detection; titrate the antibody to determine optimal concentration for your specific sample type to minimize background.
• Target expression is highest on hematopoietic cells; consider validating on mouse splenocytes, thymocytes, or peripheral blood lymphocytes.
• Include appropriate isotype controls and Fc receptor blocking steps to ensure specific staining.
• Confirm cytolytic activity modulation in NK cell assays if extending to functional studies, as the receptor’s signaling may depend on adaptor molecule expression.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-CD352/SLAMF6 antibody is part of our extensive portfolio for immune cell research; we can additionally source validated monoclonal/polyclonal antibody pairs, bulk ancillary reagents, and rare-target raw materials to support your assay development and production scale-up.