PE Rabbit anti-Mouse CD32 Monoclonal Antibody for FC - P08101

Core Product Specifications and Parameters

Biological Background: CD32 Function and Localization

• CD32, also known as low affinity immunoglobulin gamma Fc region receptor II (FcγRIIB), is a receptor for the Fc region of complexed IgG. It plays a key role in effector and regulatory functions, including phagocytosis of antigen-antibody complexes and modulation of antibody production by B-cells.
• Multiple isoforms are produced by alternative splicing: IIB1, IIB1', IIB2, and IIB3. Isoforms IIB1 and IIB1' can form caps but do not mediate endocytosis or phagocytosis, while isoform IIB2 mediates endocytosis of soluble immune complexes via clathrin-coated pits.
• CD32 belongs to the immunoglobulin gene family and contains Ig-like domains in its extracellular region. It acts as an inhibitory receptor when co‑ligated with BCR, TCR, or FcεRI, down‑regulating lymphocyte and mast cell activation.
• Subcellular localization includes the cell membrane, cytoplasm, cytoskeleton, and secreted forms. The different isoforms exhibit distinct trafficking behaviors, with IIB3 being released by macrophages and found in serum.
• CD32 is widely expressed on cells of hemopoietic origin. Isoform expression is lineage‑specific: IIB1 is preferentially expressed in lymphoid cells, IIB2 in myeloid cells, and IIB1' in both lineages and spleen cells but not in mesenteric lymph node cells.
• Post‑translational modifications include disulfide bond formation, glycosylation, and phosphorylation, which are important for its stability and signaling function.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to PE and is recommended for flow cytometry (FC). To minimize non‑specific binding, consider performing Fc receptor blocking before staining.
• The immunogen spans amino acids 30‑207 of the extracellular domain; therefore the antibody may recognize exposed epitopes on the cell surface, making it suitable for live‑cell staining.
• Due to alternative splicing, the antibody may detect multiple CD32 isoforms (predicted molecular weights 31 kDa, 33 kDa, 36 kDa). Optimize staining conditions and validate in the relevant mouse hemopoietic cell type.
• Titrate the antibody to determine the optimal concentration for your specific experimental setup and cell population.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a reliable partner for IVD raw material sourcing, providing a comprehensive portfolio from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare‑target raw materials. For your CD32/FcγRIIB immunology and inflammation research, we can streamline procurement and support your workflow from concept to clinic with technical consulting and supply chain solutions.