PE Rabbit anti-Mouse CD304/Neuropilin-1 Monoclonal Antibody for FC - P97333

Core Product Specifications and Parameters

Biological Background: Neuropilin-1 Function and Localization

• Neuropilin-1 (NRP1) is a transmembrane receptor crucial for cardiovascular development, angiogenesis, neuronal circuit formation, and organogenesis.
• It mediates the chemorepulsant activity of semaphorins, playing a key role in axon guidance (PubMed:26503042). Related references: PMID:26503042
• Recognizes a C-end rule (CendR) motif R/KXXR/K on ligands, promoting cellular internalization and vascular leakage.
• Binds semaphorin 3A, PLGF-2, VEGF165, and VEGFB; co-expression with KDR enhances VEGF165 binding and chemotaxis.
• Binding to VEGFA initiates signaling required for motor neuron axon guidance and cell body migration, including caudal migration of facial motor neurons during embryogenesis (PubMed:26503042). Related references: PMID:26503042
• Regulates mitochondrial iron transport via interaction with ABCB8/MITOSUR.
• Localizes to the mitochondrial membrane, cell membrane, and cytoplasm.
• Predominantly expressed in the nervous system and classified as a glycoprotein, phosphoprotein, heparin-binding proteoglycan with disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen encompasses the entire extracellular domain (aa 22-856), covering multiple ligand-binding regions; this design may favor detection of native conformations in flow cytometry.
• For flow cytometry (FC) on mouse samples, titrate the antibody to optimize the signal-to-noise ratio and include appropriate isotype controls.
• If adapting for other applications such as immunohistochemistry or western blotting, validate performance in the relevant sample system, as epitope accessibility and protein denaturation can affect antibody binding.
• Consider pairing with a non-PE conjugated clone for multi-parameter analysis or functional assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw material procurement for diagnostic manufacturers and research labs by offering validated antibody products, custom sourcing, and technical consultation from concept to clinic. This anti-Neuropilin-1 monoclonal antibody is part of our growing portfolio for neuroscience and angiogenesis research. Contact us to secure bulk reagents, matched antibody pairs, and ancillary materials tailored to your assay development needs.