PE Rabbit anti-Mouse CD301 (MGL1/2) mAb for Flow Cytometry - P49300 / Q8JZN1

Core Product Specifications and Parameters

Biological Background: C-type lectin domain family 10 member A (CLEC10A) Function and Localization

• CLEC10A, also known as macrophage galactose-type lectin (MGL) or CD301, is a transmembrane C-type lectin receptor encoded by the Clec10a gene in mice.
• It specifically binds terminal galactose and N-acetylgalactosamine (GalNAc) residues, mediating interactions with glycoproteins on tumor cells and other targets.
• Plays a role in the recruitment of inflammatory monocytes to adipose tissue during diet-induced obesity, contributing to adipose tissue inflammation. Related references: PMID:19995956
• Contributes to host resistance against Trypanosoma cruzi infection by promoting optimal macrophage activation. Related references: PMID:25170304
• Localized on the cell membrane, expressed on activated macrophages and detected in various tissues including lymph node, spleen, lung, and heart.
• The antibody recognizes both CLEC10A (MGL1) and the closely related CLEC10A-like (MGL2) due to sequence homology (SWISS P49300 and Q8JZN1).
• Post-translational modifications include N-linked glycosylation and disulfide bond formation typical of C-type lectins.

Experimental Guidance and Technical Tips

• The antibody is supplied as a PE-conjugate, optimized for direct flow cytometry; no secondary staining is required.
• Because the target is a cell-surface receptor, standard surface staining protocols without permeabilization are suitable for viable cell analysis.
• Titrate the antibody to determine the optimal concentration for your specific cell type; a starting range of 0.5-5 µg per 10^6 cells is often tested.
• The immunogen covers amino acids 57-304, corresponding to the extracellular domain of CD301a, ensuring recognition of the native surface protein.
• Validate reactivity in mouse macrophage cell lines such as RAW 264.7, or in primary activated macrophages, as the protein is expressed on activated myeloid cells.
• For studying MGL1 vs MGL2, confirm isoform specificity with additional assays since this pan-CD301 antibody recognizes both.
• Include appropriate isotype controls and Fc blocking steps to minimize non-specific binding in flow cytometry.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with one-stop access to IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. This PE-conjugated anti-CD301 antibody supports research in macrophage biology, tumor immunity, and infectious disease. As your dedicated sourcing partner, we also offer custom procurement for rare-target reagents and technical services to streamline your project from concept to clinic.