PE Rabbit anti-Mouse CD274/PD-L1 Monoclonal Antibody for Flow Cytometry - Q9EP73

Core Product Specifications and Parameters

Biological Background: PD-L1 (CD274) Function and Localization

• PD-L1 plays a critical role in induction and maintenance of immune tolerance to self by binding to the inhibitory receptor PD-1 (PDCD1), thereby modulating T-cell activation thresholds and limiting T-cell effector responses. Related references: PMID:11238124
• Through a yet unknown activating receptor, PD-L1 may costimulate T-cell subsets that predominantly produce interleukin-10 (IL-10). Related references: PMID:11015443 PMID:12719480
• The PD-1/PD-L1 pathway is exploited by tumors to attenuate anti-tumor immunity; PD-L1 interaction with PD-1 inhibits cytotoxic T lymphocyte (CTL) effector function, and blocking this pathway can reverse T-cell exhaustion and normalize anti-tumor responses, providing a rationale for cancer immunotherapy. Related references: PMID:12218188 PMID:27281199
• PD-L1 (CD274), also known as B7 homolog 1 (B7-H1), is a member of the immunoglobulin superfamily and contains immunoglobulin-like domains.
• PD-L1 is localized to the cell membrane, early endosome membrane, and recycling endosome membrane.
• Tissue expression: Highly expressed in the heart, thymus, skeletal muscle, and lung; weakly expressed in the kidney, spleen, thyroid, and liver. It is expressed on activated dendritic cells, B-cells, and macrophages, and in numerous tumor cell lines of lymphoid origin.
• Post-translational modifications include glycosylation and disulfide bond formation, as indicated by UniProt keywords (glycoprotein, disulfide bond, Ubl conjugation).

Experimental Guidance and Technical Tips

• This PE-conjugated monoclonal antibody is designed for flow cytometry and likely recognizes the native conformation of mouse PD-L1 on the cell surface, given that the immunogen corresponds to the extracellular domain (amino acids 19-235).
• For flow cytometry, consider using appropriate isotype control antibodies and Fc receptor blocking to minimize nonspecific binding. Titrate the antibody for optimal signal-to-noise ratio in your experimental system.
• As a PE conjugate, ensure proper compensation controls when combined with other fluorophores in multicolor panels.
• Validate staining with relevant mouse cell lines or primary cells known to express PD-L1 (e.g., activated immune cells or tumor cell lines).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. For PD-L1 and immuno-oncology research, we offer streamlined access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Our integrated supply capabilities help accelerate your assay development and diagnostic commercialization.