PE Rabbit anti-Mouse CD268/BAFF-R Monoclonal Antibody for Flow Cytometry - Q9D8D0

Core Product Specifications and Parameters

Biological Background: CD268/BAFF-R Function and Localization

• CD268/BAFF-R is a B-cell surface receptor specific for TNFSF13B (BAFF/BLyS), playing a critical role in mature B-cell survival and the humoral immune response.
• As a TNF receptor superfamily member, it mediates signals essential for B-cell maturation and B-cell homeostasis.
• The protein is a single-pass type III transmembrane receptor; it lacks a signal peptide and contains an intracellular domain that recruits TRAF adaptors.
• Subcellular localization is primarily at the plasma membrane, consistent with its role in ligand binding and signal transduction.
• Tissue expression is highest in spleen and testis, with lower levels in lung and thymus, reflecting its importance in immune and reproductive tissues.
• Post-translational modifications include disulfide bond formation and N-linked glycosylation, typical for membrane receptors.
• The mouse gene Tnfrsf13c maps to chromosome 15 and undergoes alternative splicing, generating multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (residues 10-71) of mouse CD268, which contains the BAFF-binding region; this may enhance specificity for native receptor detection.
• This PE-conjugated antibody is suitable for direct flow cytometry; consider titrating to determine optimal staining concentration for your cell type.
• For FC, use appropriate isotype controls and live/dead discrimination to accurately gate B-cell populations.
• Cross-reactivity has been validated in mouse; for other species, check sequence homology of the immunogen region before use.
• Since CD268 is expressed on B cells, consider co-staining with B-cell markers (e.g., CD19, B220) to identify specific subsets.
• The antibody detects an epitope within amino acids 10-71; it may recognize both wild-type and spliced isoforms containing this segment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, bulk reagents, and personalized sourcing for rare targets. This CD268/BAFF-R monoclonal antibody supports immunology and B-cell biology research, and CamelBio can assist with custom conjugation, scale-up, and assay development services to accelerate your diagnostic projects.