PE Rabbit anti-Mouse CD267/TACI Monoclonal Antibody for Flow Cytometry - Q9ET35

Core Product Specifications and Parameters

Biological Background: CD267/TACI Function and Localization

• CD267/TACI (Tnfrsf13b) is a member of the tumor necrosis factor receptor superfamily, also known as Transmembrane Activator and CAML Interactor.
• It functions as a high-affinity receptor for both TNFSF13/APRIL and TNFSF13B/BAFF, crucial for B-cell survival and humoral immune responses.
• Upon ligand binding, TACI activates multiple signaling cascades, including NF-kappa-B, NF-AT, and AP-1, through calcineurin-dependent pathways (By similarity).
• TACI is a transmembrane protein located on the plasma membrane, featuring a conserved cysteine-rich domain for ligand interaction and a signaling cytoplasmic tail.
• Keywords associated with the protein include adaptive immunity, disulfide bond, signal-anchor, and repeat, consistent with its receptor function and structural properties.
• TACI plays a role in regulating B- and T-cell function, and mutations or dysregulation are linked to common variable immunodeficiency (CVID) and autoimmune conditions.
• The mouse TACI protein has a predicted molecular weight of approximately 27 kDa.

Experimental Guidance and Technical Tips

• For flow cytometry, use a PE-conjugated isotype control to assess non-specific background staining.
• Titrate the antibody to determine the optimal concentration for your specific cell type and staining protocol, as conditions may vary.
• Prepare single-cell suspensions and ensure viability exclusion (e.g., with 7-AAD or DAPI) to avoid dead cell autofluorescence.
• If performing intracellular staining, include appropriate fixation and permeabilization steps compatible with the PE fluorophore.
• Validate the antibody in the relevant mouse model or tissue, and consider that cross-reactivity with other species has not been evaluated.
• This monoclonal antibody may be suitable for detecting surface TACI on activated B cells and T cells in spleen, lymph node, and blood samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes streamlined access to IVD raw materials, including high-quality monoclonal antibodies like this anti-CD267/TACI clone. Whether you need validated antibody pairs, optimized conjugates, bulk ancillary reagents, or assistance sourcing rare targets for B-cell immunity research, our comprehensive supply network can support your flow cytometry applications. Contact our team to discuss your specific procurement needs and technical requirements.