PE Rabbit anti-Mouse CD262/TRAILR2 Monoclonal Antibody for FC - Q9QZM4

Core Product Specifications and Parameters

Biological Background: CD262/TRAILR2 Function and Localization

• Tumor necrosis factor receptor superfamily member 10B (Tnfrsf10b), also known as Death receptor 5 (DR5), MK, or KILLER, is a receptor for the cytotoxic ligand TNFSF10/TRAIL.
• Upon TRAIL binding, DR5 recruits the adapter molecule FADD and caspase-8, forming the death-inducing signaling complex (DISC) that activates caspase-8 and triggers the caspase cascade leading to apoptosis.
• DR5 also promotes activation of NF-κB, a transcription factor involved in immune and inflammatory responses, and is essential for ER stress-induced apoptosis.
• The protein is a transmembrane receptor located on the cell membrane, containing a signal peptide, extracellular TRAIL-binding domains, a transmembrane helix, and a cytoplasmic death domain.
• It is a glycoprotein with disulfide bonds and belongs to the TNF receptor family, characterized by cysteine-rich repeats in the extracellular region.
• Highly expressed in mouse heart, lung, and kidney, indicating potential roles in these organs.
• UniProt keywords include: Apoptosis, Disulfide bond, Glycoprotein, Membrane, Receptor, Repeat, Signal, Transmembrane, Transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 53–170 of mouse CD262/TRAILR2, covering part of the extracellular domain, suggesting the antibody likely targets an extracellular epitope.
• Being PE-conjugated, this antibody is readymade for direct flow cytometry; consider titrating the antibody for optimal signal-to-noise ratio and using appropriate isotype controls.
• For flow cytometry, validate the antibody in a relevant mouse cell line or primary cells known to express DR5 (e.g., heart, lung, or kidney tissues).
• Include proper fixation and permeabilization steps if intracellular staining is needed, though the antibody is optimized for surface staining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to provide a comprehensive range of IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies for targets like TRAILR2 in apoptosis research. We also supply bulk ancillary reagents and offer rare-target sourcing to streamline your workflow. Connect with us to accelerate your assay development with reliable, research-grade materials.