PE Rabbit anti-Mouse CD25 Monoclonal Antibody for FC - P01590

Core Product Specifications and Parameters

Biological Background: Interleukin-2 receptor subunit alpha Function and Localization

• CD25, also known as IL-2 receptor subunit alpha (Il2ra) or p55, is encoded by the Il2ra gene in mice.
• It functions as the alpha chain of the high-affinity interleukin-2 receptor, binding IL-2 and mediating downstream signaling.
• CD25 is critically involved in immune tolerance by controlling regulatory T cell (Treg) activity, suppressing auto-reactive T-cell activation and expansion.
• The protein is a transmembrane glycoprotein located on the cell surface (membrane), featuring sushi repeat domains and a disulfide bond structure.
• Post-translational modifications include glycosylation and disulfide bond formation, essential for proper folding and receptor function.
• Keywords from UniProt indicate it contains a signal peptide, transmembrane helix, and belongs to the type I cytokine receptor family.
• The calculated molecular weight of the mouse CD25 protein is approximately 30.7 kDa (UniProt: P01590).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 22–236) of mouse CD25, which may ensure recognition of the native cell surface receptor.
• For flow cytometry (FC), titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions.
• Include appropriate controls such as isotype-matched PE-conjugated antibodies and unstained cells to assess non-specific binding.
• Consider using EDTA-containing buffers during cell preparation to prevent receptor shedding or aggregation.
• Validate the antibody’s performance in the relevant sample system, such as mouse splenocytes or lymph node cells, to confirm expected Treg staining patterns.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with access to a comprehensive range of IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents. Our one-stop sourcing platform supports immunology and immunotherapy research by supplying high-quality CD25 antibodies and related products for Treg analysis. With expertise from concept to clinic, CamelBio streamlines procurement for reliable, bulk-scale IVD material needs.