PE Rabbit anti-Mouse CD24 Monoclonal Antibody for Flow Cytometry - P24807

Core Product Specifications and Parameters

Biological Background: CD24 Function and Localization

• CD24, also known as heat stable antigen (HSA) or nectadrin, is a glycosylphosphatidylinositol (GPI)-anchored cell surface sialoglycoprotein.
• It is expressed in lymphoid, myeloid, and erythroid cells and may play a pivotal role in cell differentiation of various cell types, including early thymocyte development.
• Signaling is thought to be triggered by lectin-like ligand binding to CD24 carbohydrates, with signal transduction via release of second messengers from the GPI-anchor.
• In association with SIGLEC10, CD24 may be involved in selective suppression of immune responses to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70, and HSP90.
  Related references: PMID:19264983
• CD24 also plays a role in the control of autoimmunity.
  Related references: PMID:20200274
• The protein is localized to the cell membrane and contains a GPI-anchor, with a predicted mature molecular weight of approximately 8 kDa (UniProt: 7.8 kDa).
• Additional post-translational modifications include glycosylation, and the protein is classified under keywords such as glycoprotein, immunity, innate immunity, and signal transduction.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a short extracellular peptide (amino acids 27-53), suggesting the antibody likely recognizes an epitope in the N-terminal region of mature CD24.
• For flow cytometry, consider using standard cell surface staining protocols with appropriate Fc blocking to minimize non-specific binding.
• Validate the antibody in relevant mouse cell lines or primary cells known to express CD24 (e.g., lymphoid, myeloid, or erythroid cells) to confirm reactivity.
• When performing multicolor flow cytometry, check for potential spectral overlap with PE and ensure proper compensation.
• Store the antibody at 2-8°C protected from light, as recommended for PE conjugates to maintain fluorescence stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated anti-CD24 antibodies for flow cytometry applications. Our portfolio includes optimized rabbit monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare immune targets. Whether you need antibody pairs for assay development or large-scale production, CamelBio bridges your concept to clinic with reliable research support.