PE Rabbit anti-Mouse CD235a/Glycophorin A mAb for FC - P14220

Core Product Specifications and Parameters

Biological Background: CD235a/Glycophorin A Function and Localization

• Glycophorin-A (CD235a, GPA) is the major intrinsic membrane protein of the erythrocyte, encoded by the Gypa gene in mice.
• It is a component of the ankyrin-1 complex, which is crucial for maintaining the stability and shape of the erythrocyte membrane.
• The N-terminal extracellular segment is heavily glycosylated and carries MN blood group receptors, highlighting its role in cell surface recognition.
• Glycophorin A is required for the high activity of the anion exchanger SLC4A1 (band 3) and may facilitate its translocation to the plasma membrane.
• Subcellular localization: Integral membrane protein, primarily on the erythrocyte surface.
• Post-translational modifications include glycosylation (sialic acid) and phosphorylation, contributing to its structural and functional roles.
• Keywords from UniProt: Direct protein sequencing, Glycoprotein, Membrane, Phosphoprotein, Reference proteome, Sialic acid, Transmembrane, Transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain (aa 1-108), making this antibody suitable for detecting native CD235a on live cells in flow cytometry.
• Consider using appropriate isotype controls and Fc blocking to ensure specific staining.
• For mouse samples, cross-reactivity is confirmed; validate staining in the relevant erythroid cell type or tissue.
• The PE conjugate allows direct detection without the need for a secondary antibody, reducing assay complexity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering rapid access to validated antibodies, bulk ancillary reagents, and rare target materials. This PE-conjugated anti-mouse CD235a/Glycophorin A monoclonal antibody supports erythrocyte membrane research and MN blood group studies, and we can supply it in quantities tailored to your production or development needs. Reach out to explore our full portfolio of optimized monoclonal and polyclonal antibodies and integrated technical consulting services.