PE Rabbit anti-Mouse CD23 Monoclonal Antibody for FC - P20693

Core Product Specifications and Parameters

Biological Background: CD23 Function and Localization

• CD23, also known as Fc-epsilon-RII or lymphocyte IgE receptor, is a low-affinity receptor for immunoglobulin E (IgE) encoded by the Fcer2 (Fcer2a) gene in mice. Alternative splicing generates isoforms, including membrane-bound and secreted forms.
• It plays an essential role in regulating IgE production and B cell differentiation. On B cells, CD23 initiates IgE-dependent antigen uptake and presentation to T cells, influencing humoral immune responses.
• On macrophages, IgE binding and antigen cross-linking trigger intracellular killing of parasites via the L-arginine-nitric oxide pathway, linking adaptive and innate immunity.
• CD23 localizes primarily to the cell membrane, but a soluble form can be secreted, functioning as a soluble receptor.
• The protein is a type II transmembrane glycoprotein with a C-type lectin domain, containing disulfide bonds, N-glycosylation sites, and palmitoylation, which may modulate its activity and stability.
• CD23 expression is predominantly found on B lymphocytes and macrophages, consistent with its roles in antigen presentation and effector functions.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fusion protein spanning amino acids 50–331 of mouse CD23, which includes the extracellular domain critical for IgE binding. This design may favor recognition of native conformations.
• For flow cytometry, consider titrating the antibody to determine the optimal staining concentration for your specific cell type. Validate in appropriate mouse B cell or macrophage line models or primary cells.
• As a PE-conjugated antibody, it is directly suitable for one-step staining without secondary detection, minimizing background. Ensure proper compensation settings when used in multicolor panels.
• Since CD23 is also shed as a soluble form, note that staining might detect membrane‑bound forms; include appropriate controls if soluble CD23 could interfere.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic developers and research laboratories with streamlined access to premium IVD raw materials. This PE-conjugated anti-mouse CD23 monoclonal antibody exemplifies our supply capabilities in immunology and inflammation-related targets, supported by our range of validated antibody pairs, recombinant proteins, and customizable bulk reagents. For rare or niche target sourcing, CamelBio provides tailored procurement solutions to accelerate your assay development from concept to clinic.