PE Rabbit anti-Mouse CD206/MMR Monoclonal Antibody - Q61830

Core Product Specifications and Parameters

Biological Background: CD206/MMR Function and Localization

• CD206/MMR, encoded by the Mrc1 gene, is a type I transmembrane glycoprotein and a member of the mannose receptor family, functioning as a pattern recognition receptor.
• It mediates the endocytosis of glycoproteins by macrophages, binding both sulfated and non-sulfated polysaccharide chains.
• Acts as a phagocytic receptor for a broad range of pathogens, including bacteria, fungi, and other microorganisms, contributing to innate immune defense.
• Subcellularly, it traffics between the cell membrane and endosomal compartments, cycling to deliver ligands for degradation or antigen presentation.
• Contains an extracellular region with multiple C-type lectin domains and a fibronectin type II domain, along with a short cytoplasmic tail involved in internalization signals.
• Detected specifically in macrophages, consistent with its role in tissue surveillance and pathogen clearance.
• Key structural features include calcium-dependent carbohydrate recognition, intrachain disulfide bonds, and multiple transmembrane helices, which stabilize its functional conformation.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 20-154) targets an extracellular domain, suitable for surface receptor detection by flow cytometry without permeabilization.
• For intracellular staining (FC intra), optimize permeabilization conditions using appropriate buffers (e.g., saponin-based) to detect intracellular pools.
• Since the antibody is PE-conjugated, protect from light during incubation and storage to maintain fluorophore stability.
• Consider using a viability dye to exclude dead cells from flow cytometry analysis, as macrophages can exhibit autofluorescence.
• Validate the antibody in the relevant mouse macrophage cell lines (e.g., RAW 264.7) or primary macrophage cultures before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For immunology and infectious disease applications, we offer this PE Rabbit anti-Mouse CD206/MMR mAb as part of our growing portfolio of macrophage and innate immunity markers. As your sourcing partner, CamelBio also assists in procuring rare-target antibodies and custom reagent development to accelerate your assay development from concept to clinic.