PE Rabbit anti-Mouse CD204 Monoclonal Antibody for FC - P30204

Core Product Specifications and Parameters

Biological Background: CD204 (MSR1) Function and Localization

• CD204, also known as Macrophage scavenger receptor types I and II (MSR1), is a membrane receptor generated by alternative splicing, containing coiled-coil and collagen-like domains (UniProt P30204).
• Recognizes and internalizes modified low-density lipoproteins such as acetylated LDL (AcLDL) and oxidized LDL (OxLDL), promoting cholesterol uptake and contributing to foam cell formation during atherogenesis.
• Plays a critical role in innate immune defense by binding a broad range of pathogen-associated and damage-associated molecules, including bacterial cell wall components and apoptotic cell debris; upon CpG DNA stimulation, it mediates LYN-SYK-TLR9 signaling to enhance immune responses. Related references: PMID:9348300, PMID:10722588
• Subcellularly localized to the membrane, where it functions as an endocytic and phagocytic receptor.
• Expressed in monocyte-derived macrophages, consistent with its role in macrophage-mediated clearance and lipid metabolism.
• Post-translational modifications include glycosylation and phosphorylation; structural features include disulfide bonds and a signal-anchor transmembrane region.

Experimental Guidance and Technical Tips

• This PE-conjugated recombinant rabbit monoclonal antibody is designed for flow cytometry (FC) applications, enabling direct detection of mouse CD204 on the cell surface.
• The immunogen corresponds to amino acids 350-458 of mouse CD204, an extracellular region, supporting recognition of the native receptor on live cells. Consider proper cell surface staining protocols with appropriate Fc blocking.
• Validate the antibody’s performance using relevant macrophage cell lines or primary mouse macrophages. Perform titration to determine optimal concentration for your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, streamlining the transition from concept to clinical application. For antibodies targeting CD204 and macrophage scavenger receptors, we offer a range of monoclonal and polyclonal options, including PE-conjugated formats, validated antibody pairs, and bulk ancillary reagents to support your immunology and cardiovascular disease research. Our team can also assist in sourcing rare target raw materials, ensuring your projects proceed without supply chain interruptions.