PE Rabbit anti-Mouse CD162/PSGL-1 Monoclonal Antibody for FC - Q3TA56

Core Product Specifications and Parameters

Biological Background: CD162/PSGL-1 Function and Localization

• The protein is designated CD162/PSGL-1 and is encoded by the Selplg gene (also known as Selpl) in mouse (Mus musculus), corresponding to UniProt entry Q3TA56.
• According to UniProt, this entry is annotated as a membrane protein, consistent with its role as a cell surface receptor.
• It features a signal peptide and at least one predicted transmembrane helix, supporting its localization to the plasma membrane.
• The calculated molecular weight is approximately 43.9 kDa, in agreement with the 44 kDa observed.
• Proteomics identification keyword indicates that the protein has been detected in high-throughput mass spectrometry studies.
• As a TrEMBL entry, functional annotation is not yet available; however, orthologous proteins are known to function as selectin ligands mediating leukocyte adhesion.

Experimental Guidance and Technical Tips

• The immunogen, a recombinant fusion protein spanning amino acids 18-320 of mouse CD162/PSGL-1, covers the extracellular domain, likely preserving native conformational epitopes.
• This PE-conjugated monoclonal antibody is designed for flow cytometry; for optimal staining, titrate the antibody on your target cell population.
• Since the antibody is specific to mouse (cross reactivity: mouse), validate performance using mouse leukocyte cell lines or primary cells known to express PSGL-1.
• Consider including appropriate isotype controls and Fc receptor blocking steps to minimize non-specific binding in FC assays.
• Store at 4°C protected from light and avoid freeze-thaw cycles to maintain conjugate stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a one-stop sourcing platform for IVD raw materials, covering everything from validated antibody pairs to specialized reagents. This PE-conjugated anti-mouse CD162 monoclonal antibody exemplifies our portfolio for immunology and inflammation research applications. We also provide bulk supply and customized sourcing solutions to streamline your development workflow from early-stage R&D to clinical application.