PE Rabbit anti-Mouse CD155/Pvr Monoclonal Antibody for Flow Cytometry - Q91WP1

Core Product Specifications and Parameters

Biological Background: CD155/Pvr Function and Localization

• CD155/Pvr, encoded by the Pvr gene (also known as cd155 or D7Ertd458e), is annotated as a cell membrane protein.
• The protein belongs to the receptor category and contains a signal peptide, indicating its translocation into the endoplasmic reticulum and eventual targeting to the cell surface.
• A transmembrane helix is present, confirming its integration into the plasma membrane.
• Post-translational modifications include glycosylation (as indicated by the glycoprotein keyword) and the presence of a disulfide bond, which may stabilize the extracellular domain.
• The calculated molecular weight of 44,655 Da is consistent with the experimentally observed size of approximately 45 kDa, suggesting limited additional processing beyond glycosylation.

Experimental Guidance and Technical Tips

• This PE-conjugated monoclonal antibody is designed for direct flow cytometry without the need for a secondary reagent, simplifying the staining protocol.
• Since the immunogen corresponds to the extracellular region (amino acids 29-348), the antibody is expected to recognize the native configuration of CD155 on the cell surface.
• For optimal staining, consider titrating the antibody in the range of 0.25-1.0 µg per 10^6 cells, with incubation for 30 minutes at 4°C in the dark.
• Validate the antibody's performance in mouse cell lines known to express CD155, such as NIH/3T3 fibroblasts or mouse immune cells, and include an appropriate isotype control.
• Due to PE conjugation, handle the antibody with minimal light exposure and use a flow cytometer equipped with a blue (488 nm) or green (532 nm) laser for excitation and 575/26 bandpass filter for detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers, research labs, and institutes, offering one-stop access to IVD raw materials, technical support, and consulting from concept to clinical application. For projects involving immune checkpoint or cell adhesion targets like CD155, we can supply validated antibody pairs, optimized monoclonal antibodies (including conjugated formats), and bulk ancillary reagents to streamline your assay development. Our flexible procurement solutions also cover rare-target raw materials, customized conjugation services, and large-scale production, ensuring your supply chain remains robust and cost-effective.