PE Rabbit anti-Mouse CD138/Syndecan-1 Monoclonal Antibody for Flow Cytometry - P18828

Core Product Specifications and Parameters

Biological Background: Syndecan-1 Function and Localization

• Syndecan-1 (CD138) is a cell surface proteoglycan that contains both heparan sulfate and chondroitin sulfate, linking the cytoskeleton to the interstitial matrix (PubMed:8163535). Related references: PMID:8163535
• It regulates exosome biogenesis in concert with SDCBP and PDCD6IP (ALIX) (By similarity).
• Syndecan-1 can induce its own expression in dental mesenchymal and epithelial cells via an MSX1-mediated pathway (PubMed:8898217). Related references: PMID:8898217
• The protein localizes to the membrane and is secreted, also found in extracellular exosomes.
• Post-translational modifications include phosphorylation, glycosylation, and the attachment of heparan sulfate chains.
• Alternative splicing produces multiple isoforms of the protein.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 23-255 of mouse Syndecan-1, covering the majority of the extracellular domain; this design is expected to yield antibodies that recognize the native conformation.
• For flow cytometry, titrate the PE-conjugated antibody to determine the optimal concentration for your specific cell type and experimental conditions. Include an isotype control to assess non-specific binding.
• Due to the shedding of Syndecan-1, staining of live cells may require consideration of membrane-bound versus soluble forms. Validate in your relevant sample system.
• Since the antibody cross-reacts with mouse targets (per specification), it is suitable for mouse cell lines and primary mouse cells. Confirm reactivity in your assay if using other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including PE-conjugated monoclonal antibodies like this anti-CD138/Syndecan-1 antibody, ideal for flow cytometry applications in immunophenotyping and exosome research. Our supply chain ensures bulk availability of validated antibody pairs, ancillary reagents, and custom sourcing for rare targets, supporting your assay development from concept to clinic.