PE Rabbit anti-Mouse CD127/IL-7Rα Monoclonal Antibody for Flow Cytometry - P16872

Core Product Specifications and Parameters

Biological Background: Interleukin-7 receptor subunit alpha Function and Localization

• Interleukin-7 receptor subunit alpha (IL-7Rα) is encoded by the Il7r gene and functions as the receptor for interleukin-7 (IL-7) and thymic stromal lymphopoietin (TSLP).
• It is a single-pass type I membrane protein localized on the cell surface, with a signal peptide and a transmembrane helix.
• Expression is predominantly found in spleen, thymus, and fetal liver, aligning with its role in lymphocyte development.
• The extracellular domain contains a WSXWS motif, and the cytoplasmic tail supports downstream signaling upon ligand binding.
• Post-translational modifications include N-glycosylation, phosphorylation, and ubiquitination, which regulate receptor stability and activity.
• UniProt lists a molecular weight of approximately 51.6 kDa, though migration may vary in SDS-PAGE due to glycosylation.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 21–239 of the extracellular domain, so the antibody is expected to recognize the native surface receptor.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and staining conditions.
• Use appropriate compensation controls when analyzing PE fluorescence in multicolor panels.
• Validate staining specificity with cells of known expression, such as mouse splenocytes or thymocytes.
• Consider including a viability dye to exclude dead cells and improve data quality.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD-grade antibodies, including validated monoclonal reagents like this PE-conjugated anti-mouse CD127/IL-7Rα antibody, ideal for flow cytometry immune profiling. As a one-stop sourcing partner, we provide bulk raw materials, custom conjugations, and ancillary reagents to support research and diagnostic development from concept to clinic.