PE Rabbit anti-Mouse CD124/IL-4Ra Monoclonal Antibody for FC - P16382

Core Product Specifications and Parameters

Biological Background: CD124/IL-4Rα Function and Localization

• CD124 (interleukin-4 receptor subunit alpha, IL-4Rα) is the ligand-binding alpha chain of the type I and type II IL-4 receptors that also binds IL-13.
• Upon IL-4/IL-13 binding, CD124 activates the JAK1/2/3-STAT6 signaling cascade, driving transcriptional programs critical for Th2 cell differentiation.
• IL-4/IL-13 signaling via CD124 regulates IgE class switching, chemokine production, and mucus secretion at sites of allergic inflammation.
• Located primarily on the cell membrane, a soluble secreted form can also be generated by alternative splicing or proteolytic cleavage.
• CD124 is a glycoprotein with intrachain disulfide bonds and phosphorylation sites; it is expressed on both Th1 and Th2 cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 26-233), which may recognize the native receptor on the cell surface, making it suitable for flow cytometry.
• As a PE-conjugated primary antibody, it enables direct single-step staining; consider titrating the antibody and using Fc receptor blocking reagents for optimal results in mouse splenocytes or cell lines.
• Validate antibody performance with appropriate isotype control and compensation in multicolor panels to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw material supplier, CamelBio bridges the gap between research needs and clinical development. For allergy and inflammation studies, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, plus capability to source rare-target raw materials like CD124/IL-4Rα. Our one-stop platform supports you from concept to clinic with tailored procurement solutions.