PE Rabbit anti-Mouse CD11a Monoclonal Antibody for FC - P24063

Core Product Specifications and Parameters

Biological Background: CD11a (Integrin alpha-L) Function and Localization

• Integrin alpha-L (CD11a), designated Cd11a, LFA-1, Ly-15, and Ly-21, is the alpha subunit of the leukocyte integrin LFA-1 (αLβ2).

• ITGAL/ITGB2 functions as a receptor for ICAM1, ICAM2, ICAM3, and ICAM4 to facilitate leukocyte-endothelial cell interactions and T-cell/neutrophil transmigration. Related references: PMID:2051027, PMID:16234355, PMID:24158516

• Contributes to cytotoxic T‑cell mediated killing and antibody‑dependent cellular cytotoxicity (ADCC) by granulocytes and monocytes, as well as natural killer cell cytotoxicity.

• Required for generation of common lymphoid progenitor cells in bone marrow, indicating a role in lymphopoiesis. Related references: PMID:25108025

• Functions as a signaling receptor for ICAM2 on resting cells, ensuring leukocyte cell-cell adhesion, and also interacts with the secreted form of ubiquitin-like protein ISG15. Related references: PMID:29100055

• Localizes to the cell membrane and membrane rafts.

• Expressed selectively on leukocytes; classified with keywords including alternative splicing, glycoprotein, phosphoprotein, disulfide bond, calcium/magnesium binding, and phagocytosis.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 149–340 within the extracellular domain of mouse CD11a, suggesting the antibody may recognize an epitope in the β-propeller region involved in ligand binding. For flow cytometry, titrate the PE conjugate on relevant mouse leukocyte populations (e.g., splenocytes, lymph node cells) and include appropriate isotype controls to assess background staining.

• As LFA-1 mediates adhesion under shear conditions, consider using dynamic assays or blocking studies to confirm functional relevance in cell-cell interaction experiments.

• Validate detection in the relevant sample system, and verify performance under intended experimental conditions (e.g., temperature, fixation) to ensure reproducibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, supporting your immunological and leukocyte biology research. For projects requiring CD11a or related adhesion molecule reagents, CamelBio streamlines sourcing, technical inquiry, and logistics to help accelerate your assay development.