PE Rabbit anti-Mouse CD117/c-Kit Monoclonal Antibody for Flow Cytometry - P05532

Core Product Specifications and Parameters

Biological Background: CD117/c-Kit Function and Localization

• CD117/c-Kit is the mast/stem cell growth factor receptor Kit, a tyrosine-protein kinase encoded by the Kit gene in mice. It functions as the receptor for stem cell factor (KITLG/SCF).
• Upon SCF binding, Kit activates multiple signaling cascades, including PI3K/AKT1, RAS/MAPK, and JAK/STAT pathways, regulating cell survival, proliferation, and differentiation.
• Kit plays critical roles in hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and melanogenesis.
• The receptor is a transmembrane glycoprotein with immunoglobulin-like domains, localized primarily at the cell membrane but also detected in the cytoplasm.
• Isoform 1 is widely expressed, including in fetal liver and bone marrow. Isoform 3 is enriched in hematopoietic stem cells. Other isoforms show tissue-specific expression patterns (e.g., spermatogonia vs. spermatids).
• Key post-translational modifications include phosphorylation, glycosylation, and ubiquitination, which regulate activity and turnover. The protein is a proto-oncogene and a member of the receptor tyrosine kinase family.

Experimental Guidance and Technical Tips

• The antibody is directly conjugated to PE, making it suitable for one-step flow cytometry staining without a secondary reagent. Protect the conjugate from light during handling and storage.
• Titrate the antibody in your specific cell system to determine the optimal concentration for flow cytometry.
• Consider Fc receptor blocking (e.g., with anti-CD16/CD32 antibodies) to minimize non-specific binding when staining mouse cells.
• Include a viability dye to exclude dead cells, which may increase background staining.
• The immunogen covers the extracellular domain (amino acids 25–523), suggesting the antibody likely recognizes the native surface receptor; validate surface staining in your experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined sourcing solution for IVD raw materials, supporting diagnostic manufacturers and research labs from early development through clinical application. For studies on hematopoiesis, stem cell biology, and mast cell function, we supply a range of validated antibodies, including PE anti-CD117/c-Kit clones, along with recombinant proteins, detection antibodies, and bulk ancillary reagents. Our team also facilitates access to rare and custom target raw materials to accelerate your assay development.