PE Rabbit anti-Mouse CD103 Monoclonal Antibody for FC - Q60677

Core Product Specifications and Parameters

Biological Background: Integrin alpha-E Function and Localization

• Integrin alpha-E (ITGAE, also known as CD103 or integrin alpha M290) pairs with integrin beta-7 (ITGB7) to form the alpha-E/beta-7 heterodimer.
• This integrin serves as a receptor for E-cadherin, mediating calcium-dependent cell adhesion.
• It plays a critical role in the adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers, facilitating mucosal immune surveillance.
• The protein is a single-pass type I membrane glycoprotein with a large extracellular domain containing integrin alpha repeats, a transmembrane helix, and a short cytoplasmic tail.
• Structurally, it features disulfide bonds, N-glycosylation sites, and metal-binding domains essential for its function.
• UniProt lists it as a member of the integrin alpha chain family with reference proteome status and signaling receptor activity.
• Expression is predominantly associated with mucosal T cells, particularly intra-epithelial lymphocytes.

Experimental Guidance and Technical Tips

• The antibody is supplied as a PE conjugate, ready for direct flow cytometry; ensure appropriate compensation and isotype controls are used.
• The immunogen spans amino acids 191–385 within the extracellular domain; consider that the epitope is likely accessible on live, non-permeabilized cells.
• Validate reactivity on mouse intra-epithelial lymphocytes or CD103-transfected cell lines to confirm specificity in your system.
• Titrate the antibody to determine the optimal staining concentration for your particular flow protocol, as PE is a bright fluorophore.
• Protect the antibody from light to prevent photobleaching and handle using appropriate precautions for fluorescent reagents.
• The target molecular weight is approximately 129 kDa; this information may aid in gating strategies or complementary techniques.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD antibodies and raw materials. This PE anti-CD103 antibody supports mucosal immunology and T-cell adhesion research, where reliable flow cytometry reagents are essential. Beyond antibodies, we offer validated antibody pairs, bulk ancillary reagents, and rare target sourcing to accelerate your assay development from concept to clinic.