PE Rabbit anti-Human/Mouse/Monkey FOXP3 mAb - Q9BZS1

Core Product Specifications and Parameters

Biological Background: FOXP3 Function and Localization

• FOXP3 (Forkhead box protein P3), also known as scurfin, is a transcription factor critical for the development and suppressive function of regulatory T-cells (Tregs). Related references: PMID:17377532, PMID:21458306, PMID:23947341, PMID:23169781

• Acts as both a transcriptional repressor and activator, modulating key immune genes such as CTLA4, TNFRSF18, IL2, and IFNG to maintain immune homeostasis. Related references: PMID:17377532, PMID:21458306, PMID:23947341

• Mediates repression of IL2 through association with histone acetylase KAT5 and histone deacetylase HDAC7. Related references: PMID:17360565

• Inhibits cytokine production by repressing the activity of transcription factors RELA and NFATC2. Related参考文献: PMID:15790681

• Interacts with RUNX1 to regulate expression of CTLA4, IL2, and IFNG. Related references: PMID:17377532

• Antagonizes RORC and RORA, thereby inhibiting Th17 differentiation and promoting Treg development. Related references: PMID:18368049, PMID:18354202

• Localizes predominantly in the nucleus, with some cytoplasmic presence.

• Mutations in FOXP3 cause IPEX (Immune dysregulation, Polyendocrinopathy, Enteropathy, X-linked) syndrome, characterized by severe autoimmunity.

Experimental Guidance and Technical Tips

• For intracellular flow cytometry, use appropriate fixation and permeabilization buffers optimized for nuclear antigens.
• Titrate the antibody to determine the optimal staining concentration for your specific cell type and experimental conditions.
• Validate species cross-reactivity with positive control cells or tissues from human, mouse, and cynomolgus.
• Include an isotype control and fluorescence-minus-one (FMO) controls to ensure specific staining.
• For Treg analysis, co-stain with CD4 and CD25 to identify the classical Treg population.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing platform for IVD raw materials, technical services, and consulting. This PE-conjugated FOXP3 antibody supports immunology research and diagnostic assay development targeting Treg cells. In addition to this product, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets to accelerate your project from concept to clinic.