PE Rabbit anti-Human/Mouse PDGFRα/CD140a Monoclonal Antibody for FC - P16234

Core Product Specifications and Parameters

Biological Background: PDGFRα/CD140a Function and Localization

• PDGFRα/CD140a is a tyrosine-protein kinase receptor for PDGFA, PDGFB, and PDGFC, also known as alpha platelet-derived growth factor receptor, CD140a antigen, and PDGFR-2.
• It plays essential roles in embryonic development, including skeletal development and cephalic closure, and regulates cell proliferation, survival, chemotaxis, and differentiation of mesenchymal stem cells.
• Localized to the cell membrane, cell projections, cilium, and Golgi apparatus, facilitating signal transduction upon ligand binding.
• Activation triggers phosphorylation of PIK3R1, PLCG1, and PTPN11, leading to AKT1, MAPK/ERK, and STAT pathway activation, and is down-regulated by phosphatases and receptor internalization.
• Widely expressed in tissues including platelets, brain, fibroblasts, smooth muscle, heart, and colon tumors, highlighting its roles in both normal physiology and oncology.
• Keywords: Phosphoprotein, glycoprotein, disulfide bond, ubl conjugation; involved in chemotaxis, developmental processes, and host-virus interactions.

Experimental Guidance and Technical Tips

• Based on the immunogen design targeting the extracellular domain (aa 24-524), this antibody is optimized for flow cytometry (FC) applications detecting surface-expressed PDGFRα.
• For FC experiments, use live, unfixed cells to maintain epitope integrity, and include appropriate isotype controls to confirm specificity.
• Cross-reacts with both human and mouse PDGFRα; validate in the relevant species cell lines or primary samples to confirm performance.
• Due to PE conjugation, avoid multiple freeze-thaw cycles and protect from prolonged light exposure to preserve fluorophore stability.
• For other applications (e.g., immunohistochemistry, western blotting), consider initial pilot testing as performance may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated sourcing partner for IVD raw materials, offering a one-stop channel to validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This PE-conjugated anti-PDGFRα antibody supports oncology and developmental biology research workflows, and our team is ready to assist with rare target raw-material sourcing and customized solutions. From concept to clinic, count on CamelBio to streamline your supply chain.