PE Rabbit anti-Human/Mouse Nanog mAb - Q80Z64

Core Product Specifications and Parameters

Biological Background: Homeobox Protein NANOG Function and Localization

• NANOG is a homeobox transcription factor that maintains embryonic stem (ES) cell self-renewal and pluripotency by regulating inner cell mass proliferation. Related references: PMID:25825768
• It prevents ES cell differentiation into extraembryonic endoderm and trophectoderm lineages, and blocks BMP-induced mesoderm differentiation through physical interaction with SMAD1.
• NANOG can function as both a transcriptional activator and repressor, binding optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'.
• In differentiating cells, NANOG autorepresses its own expression by recruiting the NuRD complex to its promoter after interaction with ZNF281/ZFP281.
• Subcellular localization is exclusively nuclear, consistent with its role as a transcription factor.
• Tissue specificity analysis shows NANOG is not expressed at the protein level in oocytes and spermatogonia, and is absent from somatic organs, ovary, testis, fibroblast, and hematopoietic cell lines.
• Alternative splicing yields multiple isoforms, and the protein contains a homeobox domain characteristic of developmental regulators.
• Keywords: Activator, Alternative splicing, Developmental protein, DNA-binding, Homeobox, Nucleus, Repressor, Transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 160–305 of mouse Nanog, a region conserved in human, supporting the reported human and mouse cross-reactivity.
• For intracellular flow cytometry, standard fixation and permeabilization protocols should be optimized; consider testing different conditions for your specific cell type.
• As a PE-conjugated monoclonal antibody, verify instrument compatibility and include appropriate isotype controls.
• Validate the antibody in the relevant stem cell or developmental model system, and confirm specificity by knockout or knockdown experiments if possible.

CamelBio: Your One-Stop Sourcing Bridge

This PE anti-Nanog antibody supports stem cell and developmental biology research, and CamelBio offers comprehensive access to IVD raw materials—including validated antibody pairs, monoclonal and polyclonal reagents, and ancillary bulk supplies—to streamline your workflow from concept to clinic. Our procurement network can also accommodate rare-target sourcing needs, ensuring you have reliable materials for diagnostic and research applications.