PE Rabbit anti-Human/Monkey PD-1/CD279 Monoclonal Antibody for FC - Q15116

Core Product Specifications and Parameters

Biological Background: PD-1/CD279 Function and Localization

• Programmed cell death protein 1 (PD-1/CD279) is an inhibitory receptor on antigen-activated T-cells that plays a critical role in maintenance of immune tolerance to self. Related references: PMID:21276005 PMID:31754127 PMID:32184441

• Upon binding to its ligands CD274/PD-L1 and CD273/PD-L2, PD-1 delivers inhibitory signals that suppress T-cell activation. Related references: PMID:21276005 PMID:26602187

• PD-1 associates with the TCR-CD3 complex in the immunological synapse and directly inhibits T-cell activation. Related references: PMID:32184441

• Following ligand binding, PD-1 is phosphorylated within the ITSM motif, leading to recruitment of PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules such as ZAP70, PRKCQ/PKCtheta, and CD247/CD3zeta. Related references: PMID:32184441

• The PD-1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity; interaction with CD274/PD-L1 inhibits cytotoxic T lymphocytes (CTLs) effector function. Related references: PMID:28951311

• Blocking the PD-1 pathway reverses the exhausted T-cell phenotype and normalizes the anti-tumor response, providing a rationale for cancer immunotherapy. Related references: PMID:22658127 PMID:25034862 PMID:25399552

• PD-1 is a single-pass type I membrane glycoprotein localized at the cell membrane, containing an immunoglobulin V-type domain.

• Post-translational modifications include phosphorylation, glycosylation, and ubiquitination, with the ITSM phosphorylation being critical for downstream signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 25–168) of human PD-1, which includes the ligand-binding region; the antibody likely recognizes the native conformation and is suitable for flow cytometry on live cells.
• Given the cross-reactivity with cynomolgus monkey, consider validating the antibody in relevant primate sample systems (e.g., PBMCs) if required.
• As a PE-conjugated antibody, ensure proper compensation controls and use of an appropriate isotype control in multicolor flow cytometry panels.
• For functional blocking applications, validate whether this clone interferes with PD-1/ligand interactions in a relevant assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. Our PD-1/CD279 portfolio supports immuno-oncology research and assay development, complemented by rare target raw-material sourcing capabilities. Contact us to simplify your procurement from concept to clinic.