PE Rabbit anti-Human/Monkey Ki67 Monoclonal Antibody for Intracellular Flow Cytometry - P46013

Core Product Specifications and Parameters

Biological Background: Ki67 Function and Localization

• Ki67 is a large nuclear protein encoded by the MKI67 gene, widely used as a cellular proliferation marker.
• It associates with the surface of mitotic chromosomes, acting as a chromosome repellent to maintain individual chromosome dispersion during early mitosis.
  Related references: PMID:27362226, PMID:32879492, PMID:35513709
• During early mitosis, Ki67 forms extended brush-like structures that provide a steric and electrostatic barrier to prevent chromosome collapse.
  Related references: PMID:27362226
• In anaphase, dephosphorylation triggers a functional switch: Ki67 becomes a chromosome attractant, promoting chromosome clustering through RNA-dependent liquid-liquid phase separation.
  Related references: PMID:28935370, PMID:39153474
• Ki67 binds DNA, with a preference for supercoiled DNA and AT-rich DNA, and may contribute to chromatin organization.
  Related references: PMID:10878551, PMID:24867636
• It localizes to the nucleus and nucleolus during interphase, and covers the chromosome surface during mitosis.
• The protein undergoes various post-translational modifications, including phosphorylation, acetylation, and ubiquitination, which regulate its mitotic functions.
• As a product of the MKI67 gene, Ki67 expression is tightly linked to cell cycle progression and is absent in quiescent cells.

Experimental Guidance and Technical Tips

• Since Ki67 is a nuclear antigen, intracellular flow cytometry requires effective fixation and permeabilization; optimize permeabilization steps for your cell type.
• The antibody is raised against a synthetic peptide in the C-terminal region (aa 2271-2370); ensure that this epitope is present in your target cell or tissue.
• Use appropriate isotype controls and compensation settings for PE-conjugated antibodies during flow cytometry.
• This antibody cross-reacts with cynomolgus monkey; validate in the relevant non-human primate samples if used in translational research.
• For proliferation studies, consider co-staining with DNA content dyes (e.g., DAPI, Hoechst) or other cell cycle markers to distinguish cell cycle phases.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes streamlined access to premium IVD raw materials, including this PE-conjugated anti-Ki67 monoclonal antibody optimized for intracellular flow cytometry. Whether you need validated antibody pairs, bulk ancillary reagents, or assistance sourcing rare-target raw materials for proliferation and oncology applications, our one-stop procurement platform bridges the gap from concept to clinic. Partner with us to accelerate your diagnostic development with reliable, high-performance reagents.