PE Rabbit anti-Human/Monkey IgM mAb for FC - P01871

Core Product Specifications and Parameters

Biological Background: IgM Function and Localization

• IGHM encodes the constant region of the immunoglobulin heavy chain mu (IgM), which exists as membrane-bound B cell receptor (BCR) and secreted antibody.
• Membrane-bound IgM functions as a BCR, recognizing specific antigens and triggering B cell activation and clonal expansion PMID:20176268 PMID:22158414.
  Related references:
• The variable domains are assembled via V(D)J recombination and undergo somatic hypermutation, enabling affinity maturation upon antigen exposure PMID:17576170 PMID:20176268.
  Related references:
• Secreted IgM (sIgM) multimerizes mainly into pentamers, conferring high-avidity antigen binding; natural sIgM are polyreactive while immune sIgM are antigen-specific PMID:32029689 PMID:37095205.
  Related references:
• sIgM mediates effector functions by binding Fc receptor FCMR on lymphoid cells and recruiting C1q to activate the classical complement pathway, promoting pathogen neutralization and clearance of apoptotic cells PMID:12847249 PMID:19006321 PMID:28230186.
  Related references:
• In mucosal immunity, sIgM is transported across epithelial barriers by PIGR and can undergo FCMR-mediated retrotranscytosis for antigen delivery to mucosal lymphoid tissues PMID:32029689.
  Related references:
• Subcellularly, IgM is both secreted and anchored on the cell membrane, consistent with its dual role in humoral immunity.
• Key post-translational modifications include glycosylation and disulfide bond formation, essential for its structure and multimerization.

Experimental Guidance and Technical Tips

• This PE-conjugated monoclonal antibody is designed for flow cytometry (FC). Titrate the antibody in the relevant sample system to determine optimal staining concentration.
• The immunogen maps to the Fc constant region (amino acids 218-453 of P01871), suggesting recognition of both soluble and membrane IgM. Verify reactivity in your assay conditions.
• Use appropriate isotype controls and Fc block to minimize non-specific binding, especially when analyzing B cells or samples with high Fc receptor expression.
• Because IgM can form high-molecular-weight multimers, ensure sample preparation prevents aggregation and use freshly prepared buffers.
• Cross-reactivity has been shown with human and cynomolgus; for other species, validate binding experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, providing one-stop sourcing from concept to clinic. This anti-IgM antibody supports immunology and B cell research, complementing our range of monoclonal antibodies, recombinant proteins, and ancillary reagents for diagnostic development. Whether you need validated antibody pairs or rare target antibodies, CamelBio ensures reliable supply and expert technical support to accelerate your projects.