PE Rabbit anti-Human/Monkey IgD Monoclonal Antibody for FC - P01880

Core Product Specifications and Parameters

Biological Background: IgD Function and Localization

• IgD (immunoglobulin heavy constant delta) is the constant region of the delta heavy chain, forming part of membrane-bound and secreted antibodies. Related references: PMID:22158414
• Membrane-bound IgD (mIgD) is co-expressed with IgM on the surface of most naïve B cells and serves as the primary antigen receptor, initiating B-cell activation and differentiation. Related references: PMID:20176268
• Upon antigen binding, mIgD triggers phosphorylation of CD79A and CD79B via Src-family tyrosine kinases, initiating downstream signaling. Related references: PMID:20176268
• Soluble IgD (sIgD) is present at low concentrations in serum and acts as a potent immunomodulator, inducing pro-inflammatory cytokines such as TNF, IL1B, and IL1RN from monocytes. Related references: PMID:10702483, PMID:11282392, PMID:8774350
• The variable domains of IgD are assembled through V(D)J recombination and can undergo somatic hypermutation, allowing affinity maturation. Related references: PMID:17576170
• IgD is found both secreted and on the cell membrane, with a single-pass transmembrane domain in the membrane-bound form.
• Additional keywords indicate that IgD is a glycoprotein with intrachain disulfide bonds and has known 3D structures.
• Alternative splicing results in membrane-bound and secreted isoforms; the immunogen corresponds to the constant region between amino acids 99-384.

Experimental Guidance and Technical Tips

• The immunogen (recombinant protein covering aa 99-384 of human IgD delta constant region) targets a conserved sequence; verify cross-reactivity in cynomolgus monkey samples as indicated.
• This PE-conjugated monoclonal antibody is validated for flow cytometry; consider titrating the antibody (e.g., 5–10 µL per test) in your specific cell system to optimize signal-to-noise ratio.
• For membrane-bound IgD detection, use gentle cell handling and avoid prolonged incubation to prevent receptor shedding or internalization.
• Combine with anti-IgM and other lineage markers (e.g., CD19, CD27) for comprehensive B-cell immunophenotyping.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a single source for IVD raw materials, from validated monoclonal antibodies like this anti-IgD clone to bulk ancillary reagents. Whether you need optimized antibody pairs for B-cell immunophenotyping or rare target sourcing, our supply chain supports your workflow from concept to clinic.