PE Rabbit anti-Human/Monkey CD8a Monoclonal Antibody for Flow Cytometry - P01732

Core Product Specifications and Parameters

Biological Background: CD8a Function and Localization

• CD8a is an integral membrane glycoprotein that serves as a coreceptor for MHC class I molecules on T-cells, recognizing peptide antigens derived from cytosolic proteins.
• It interacts simultaneously with the T-cell receptor (TCR) and MHC class I, recruiting the Src kinase LCK to initiate signaling pathways leading to T-cell activation, proliferation, and cytotoxic responses.
• In natural killer (NK) cells, CD8A homodimers at the cell surface support conjugation and lysis of multiple target cells, and promote survival and differentiation into memory CD8 T-cells.
• The protein is predominantly expressed at the cell membrane but can also be secreted. Tissue expression includes thymus-derived T-cells (usually as a heterodimer with CD8B), NK cells, memory T-cells, intraepithelial lymphocytes, monocytes, and dendritic cells.
• It is also detected on plasmacytoid dendritic cells upon herpes simplex virus-1 stimulation.
• Post-translational modifications include glycosylation, disulfide bond formation, and palmitoylation, which are important for its stability and function.
• Alternative splicing produces multiple isoforms, and mutations are associated with disease susceptibility.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (aa 22-182), making this antibody suitable for detecting native CD8a on live cells without permeabilization.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and staining conditions, and include appropriate isotype controls.
• Validate antibody performance using known positive controls (e.g., human peripheral blood T-cells) and negative controls (e.g., CD8a-negative cell lines).
• Consider using this PE-conjugated antibody in multicolor panels with other lineage markers to characterize T-cell subsets.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to IVD-critical raw materials, including validated monoclonal antibodies like this CD8a clone. Leverage our extensive supply network for bulk ancillary reagents, optimized antibody pairs, and rare-target sourcing—all designed to accelerate your immune-monitoring assay development. Let CamelBio be your procurement partner for CD8a-related research and beyond.