PE Rabbit anti-Human/Monkey CD86 Monoclonal Antibody for FC - P42081

Core Product Specifications and Parameters

Biological Background: CD86 Function and Localization

• CD86 (also known as B7-2) is a type I transmembrane protein and a member of the immunoglobulin superfamily, functioning as a costimulatory molecule essential for T-lymphocyte activation.
• It provides a primary costimulatory signal for naïve T-cells by ligating the CD28 receptor, which is constitutively expressed on T-cells, thereby enhancing T-cell receptor (TCR)-mediated signaling. Related references: PMID:12196291 PMID:7694363
• CD86 also binds the inhibitory receptor CTLA4 (cytotoxic T-lymphocyte-associated protein 4), acting as a decoy to limit CD28-mediated T-cell priming and maintain peripheral tolerance. Related references: PMID:11279501
• Within the first 24 hours of activation, CD86-mediated costimulation critically influences the fate of T-cells, determining whether they become activated or anergic. Related references: PMID:7527824
• In B cells, CD40 ligation triggers CD86 to activate the NF-kappa-B pathway via phospholipase C and protein kinase C, playing a role in immunoglobulin class switching to IgG1.
• CD86 serves as a host cell receptor for adenovirus subgroup B, mediating viral attachment and entry.
• The protein is expressed on professional antigen-presenting cells such as dendritic cells, macrophages, and B cells, consistent with its role in initiating adaptive immune responses.
• Post-translational modifications include glycosylation and ubiquitination, which may regulate its stability and surface expression.

Experimental Guidance and Technical Tips

• When using this PE-conjugated antibody in flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions to minimize background and maximize signal-to-noise ratio.
• The immunogen covering the extracellular domain (amino acids 20–241) suggests the antibody should recognize native surface CD86; confirm specificity using appropriate positive and negative cell controls.
• For multicolor flow cytometry panels, use single-stained compensation controls to correct for PE fluorescence spillover, and include a PE-labeled isotype control to assess nonspecific binding.
• Cross-reactivity with cynomolgus CD86 has been confirmed; if applying this reagent to other non-human primate species, validate binding in your own assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostics manufacturers and research laboratories with a one-stop portfolio of IVD raw materials, including validated monoclonal antibodies, recombinant proteins, and ancillary reagents for immunology and cell biology applications. This CD86 antibody supports flow cytometric analysis of T-cell costimulatory pathways. For custom requirements or bulk procurement, our sourcing team can assist with specialized targets and supply chain solutions to accelerate your assay development.