PE Rabbit anti-Human/Monkey CD64 Monoclonal Antibody for Flow Cytometry - P12314

Core Product Specifications and Parameters

Biological Background: CD64 Function and Localization

• CD64, also known as Fc-gamma RI (FcγRI) or FCGR1A, is the high-affinity receptor for the Fc region of immunoglobulin gamma (IgG). It plays a critical role in both innate and adaptive immune responses.
• It mediates IgG effector functions on monocytes, triggering antibody-dependent cellular cytotoxicity (ADCC) against virus-infected cells.
• CD64 is a member of the immunoglobulin superfamily and contains immunoglobulin-like domains, a transmembrane helix, and a signal peptide.
• It is specifically expressed on monocytes and macrophages, highlighting its role in phagocytic immune responses.
• The protein undergoes post-translational modifications including glycosylation and phosphorylation, which may regulate its function and signaling.
• Alternative splicing produces multiple isoforms, potentially modulating receptor activity and expression.
• CD64 functions as a key link between humoral and cellular immunity by binding IgG-opsonized targets.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (amino acids 16-288), making this antibody suitable for detecting native surface CD64 in flow cytometry applications.
• For optimal staining, consider using an Fc receptor blocking step to minimize non-specific binding to other Fcγ receptors.
• Validate the antibody in relevant cell systems, such as monocyte/macrophage cell lines or primary human monocytes, which express high levels of CD64.
• As a PE-conjugated antibody, it provides bright fluorescence; titrate the antibody for each experimental setup to achieve the best signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, contract research organizations, and academic laboratories with a comprehensive portfolio of IVD raw materials. For CD64-targeted immunology and ADCC research, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our one-stop sourcing model streamlines procurement for rare targets, helping you accelerate assay development from concept to clinic.