PE Rabbit anti-Human/Monkey CD45 mAb - P08575

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45, also known as leukocyte common antigen (L-CA) and T200, is a receptor-type tyrosine-protein phosphatase C encoded by the PTPRC gene.
• It is essential for T-cell activation via the antigen receptor, acting as a positive regulator of coactivation upon DPP4 binding.
  Related references: PMID:35767951
• The protein contains two phosphatase domains; the first has enzymatic activity, and the second modulates substrate specificity. It dephosphorylates key signaling molecules such as SKAP1, FYN, and LYN.
• CD45 is a single-pass type I membrane protein located in membrane rafts and at the immunological synapse.
• Multiple isoforms (1–8) are generated by alternative splicing, with tissue-specific expression; isoforms 1,2,3,5,7 are detected in thymocytes.
• It plays a role in modulating immune signaling, including interaction with CLEC10A to limit autoreactivity, and can act as a receptor for human cytomegalovirus UL11, impairing T-cell proliferation.
• Post-translational modifications include glycosylation and phosphorylation; the protein belongs to the protein phosphatase family and is linked to severe combined immunodeficiency (SCID) disease variants.

Experimental Guidance and Technical Tips

• This PE‑conjugated monoclonal antibody is optimized for direct flow cytometry detection of surface CD45 on live cells. Consider titrating the antibody to determine the optimal concentration for your experimental system.
• Include appropriate isotype controls and fluorescence-minus-one (FMO) controls to ensure specificity.
• The immunogen covers the extracellular region (aa 26–577), so the antibody recognizes surface‑expressed CD45. Confirm surface staining using a viability dye to exclude dead cells.
• Cross‑reactivity with cynomolgus CD45 has been reported; validate performance in the relevant non‑human primate samples if needed.
• CD45 isoforms have varying molecular weights (131–147 kDa); migrate accordingly in WB as a positive control, though this product is validated only for FC.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic laboratories, researchers, and in vitro diagnostic manufacturers with streamlined access to high‑quality IVD raw materials and technical services—from concept to clinic. This anti‑CD45 monoclonal antibody supports immune cell profiling and T‑cell activation studies, and CamelBio can assist with sourcing validated antibody pairs, bulk ancillary reagents, and rare‑target raw materials tailored to your assay development needs.