PE Rabbit anti-Human/Monkey CD4 Monoclonal Antibody for Flow Cytometry - P01730

Core Product Specifications and Parameters

Biological Background: CD4 Function and Localization

• Encoded by the CD4 gene, T-cell surface glycoprotein CD4 (also known as T4, Leu-3) is an integral membrane glycoprotein with a molecular weight of approximately 51 kDa, containing immunoglobulin-like domains.
• Functions as a coreceptor for MHC class II molecules on antigen-presenting cells, interacting simultaneously with the T-cell receptor (TCR) and MHC class II to enhance T-cell activation.
• Recruits the Src kinase LCK to the TCR-CD3 complex, initiating intracellular signaling cascades that promote lymphokine production, motility, adhesion, and T-helper cell activation.
• In macrophages and NK cells, CD4 participates in differentiation, activation, cytokine expression, and cell migration through TCR/LCK-independent pathways; it also triggers monocyte differentiation into mature macrophages.
• Plays a critical role in thymic development of T-helper cells and is highly expressed on T-helper cells, with additional presence on macrophages, dendritic cells, and NK cells.
• Serves as the primary receptor for human immunodeficiency virus-1 (HIV-1), with viral entry mediated by gp120 binding; additionally acts as a receptor for Human Herpesvirus 7 (HHV-7). HIV-1 Vpu protein down-regulates CD4 expression. Related references: PMID:12089508 PMID:16331979 PMID:2214026 PMID:17346169 PMID:7909607
• Subcellularly localized to the cell membrane, CD4 is subject to post-translational modifications including disulfide bond formation, glycosylation, palmitoylation, and phosphorylation, which regulate its function and interactions.

Experimental Guidance and Technical Tips

• This PE-conjugated monoclonal antibody (clone not specified) was generated using recombinant human CD4 protein as immunogen and is validated for flow cytometry (FC) applications.
• Since the antibody is pre-conjugated with phycoerythrin (PE), avoid prolonged exposure to light and follow standard FC protocols for cell staining and acquisition; a titration experiment is recommended to determine the optimal working concentration.
• The antibody cross-reacts with human and cynomolgus CD4; if using other species, confirm cross-reactivity in the relevant sample system before proceeding with critical experiments.
• For intracellular staining, a fixation/permeabilization step may be required; the antibody is suitable for surface staining of live cells without fixation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is an integrated supplier supporting IVD manufacturers and research institutions with raw materials, technical services, and consulting from concept to clinical application. This PE-conjugated anti-CD4 monoclonal antibody is part of our portfolio of validated immune cell marker reagents, ideal for T-helper cell immunophenotyping, HIV receptor studies, and immune monitoring. In addition to labeled primary antibodies, CamelBio provides optimized antibody pairs, bulk ancillary reagents, and sourcing assistance for rare-target raw materials—streamlining your procurement and accelerating diagnostic development.