PE Rabbit anti-Human/Monkey CD3 mAb for FC - P07766

Core Product Specifications and Parameters

Biological Background: CD3 Function and Localization

• CD3E encodes the CD3 epsilon chain, a core component of the T-cell receptor (TCR)-CD3 complex on the surface of T lymphocytes, essential for adaptive immunity. Related references: PMID:15294938, PMID:15546002, PMID:2470098
• Upon TCR engagement by antigen-presenting cells, CD3 chains transmit activation signals via immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domains, which are phosphorylated by LCK and FYN to recruit and activate ZAP70. Related references: PMID:2470098, PMID:40592325
• Beyond signal transduction, CD3E is required for proper T-cell development and maturation.
• CD3E also regulates TCR-CD3 complex internalization and cell surface down-regulation through endocytosis sequences in its cytoplasmic tail. Related references: PMID:10384095, PMID:26507128
• Additionally, CD3E acts as a receptor for ITPRIPL1, providing an inhibitory signal that recruits NCK1, blocks CD3E–ZAP70 interaction, and suppresses ERK-NFkB signaling and calcium influx, thereby modulating T-cell activation. Related references: PMID:12110186, PMID:38614099
• The protein is a single-pass type I membrane protein localized to the cell membrane, with an extracellular immunoglobulin-like domain, a transmembrane helix, and a cytoplasmic tail containing ITAMs.
• CD3E is also known by aliases T3E, TCRE, and IMD18, and belongs to the immunoglobulin superfamily.

Experimental Guidance and Technical Tips

• As a PE-conjugated antibody, this product is designed for flow cytometry; ensure appropriate light protection during staining and acquisition.
• When evaluating cross-reactivity with cynomolgus CD3, consider parallel staining of human and cynomolgus samples to confirm reactivity in your specific experimental system.
• Include suitable isotype and fluorescence-minus-one (FMO) controls to set gates and assess background staining.
• Titrate the antibody to determine the optimal concentration that achieves maximal signal-to-noise ratio for your cellular target and instrument.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research institutions with premium-quality IVD raw materials, including monoclonal antibodies, validated antibody pairs, and bulk ancillary reagents. This PE-conjugated anti-CD3 antibody supports T-cell immunophenotyping and adaptive immunity research, and we offer scalable supply solutions alongside technical consulting for assay development. For hard-to-find targets or custom sourcing needs, CamelBio provides comprehensive procurement support from concept to clinic.