PE Rabbit anti-Human/Monkey CD25 Monoclonal Antibody for Flow Cytometry - P01589

Core Product Specifications and Parameters

Biological Background: CD25 Function and Localization

• Interleukin-2 receptor subunit alpha (CD25) serves as a receptor for interleukin-2 (IL-2).
• CD25 is essential for immune tolerance by regulating regulatory T cell (Treg) activity; Tregs suppress autoreactive T-cell activation and expansion.
• The protein is a membrane-bound glycoprotein with a signal peptide, extracellular sushi domains, a single transmembrane helix, and intramolecular disulfide bonds.
• CD25 is associated with disease variants involved in diabetes mellitus and other immune disorders.
• The predicted molecular weight of mature CD25 is approximately 30.8 kDa, consistent with the observed ~31 kDa band.

Experimental Guidance and Technical Tips

• The immunogen (recombinant protein covering amino acids 22–213) corresponds to the extracellular domain, suggesting the antibody may recognize native CD25 conformation—ideal for flow cytometry.
• For FC experiments, titrate the antibody on target cells and include appropriate isotype and compensation controls, especially when using PE conjugates.
• Consider co-staining with CD4 and FoxP3 to identify Treg populations in human or cynomolgus peripheral blood or tissue samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with reliable, one-stop access to IVD raw materials, including this PE-conjugated CD25 monoclonal antibody. Our portfolio spans validated antibody pairs, bulk ancillary reagents, and rare-target sourcing to accelerate your immune monitoring and tolerance research workflows.