PE Rabbit anti-Human/Monkey CD20 Monoclonal Antibody for FC - P11836

Core Product Specifications and Parameters

Biological Background: CD20 Function and Localization

• CD20, also known as MS4A1, Bp35, and Leu-16, is a member of the membrane-spanning 4A (MS4A) protein family.
• It serves as a store-operated calcium (SOC) channel component, mediating calcium influx downstream of B-cell receptor (BCR) activation, which is essential for B-lymphocyte development, differentiation, and activation. Related references: PMID:12920111 PMID:3925015 PMID:7684739
• CD20 is a multi-pass transmembrane protein localized to the cell membrane.
• Expression of CD20 is restricted to B-lymphocytes.
• The protein undergoes post-translational modifications including disulfide bond formation, palmitoylation, and phosphorylation.
• Alternative splicing of the MS4A1 gene yields multiple transcript variants encoding different isoforms.
• CD20 is a clinically validated pharmaceutical target, with therapeutic antibodies widely used in treating B-cell malignancies and autoimmune disorders.

Experimental Guidance and Technical Tips

• The antibody is generated against a synthetic peptide corresponding to amino acids 100–200 of human CD20; it may recognize a linear epitope.
• PE conjugation makes it suitable for direct flow cytometry analysis without the need for a secondary antibody.
• Consider titrating the antibody to determine the optimal concentration for your specific sample type (e.g., human PBMCs, cynomolgus lymphocytes).
• Validate staining in your experimental system, as fixation and permeabilization steps may affect epitope accessibility.
• For multicolor flow cytometry panels, ensure minimal spectral overlap between PE and other fluorophores.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials. This PE-conjugated anti-CD20 antibody supports B-cell analysis in flow cytometry-based diagnostic and research applications. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to streamline your assay development from concept to clinic.